Epidermal Growth Factor Modulates Palmitic Acid-Induced Inflammatory and Lipid Signaling Pathways in SZ95 Sebocytes

Epidermal growth factor (EGF) acts as a paracrine and autocrine mediator of cell proliferation differentiation in various types epithelial cells, such sebocytes, which produce the lipid-rich sebum to moisturize skin. However, lipids via direct contact by penetrating through epidermis may have regulatory roles on epidermal dermal cells well. As EGF receptor (EGFR) is expressed throughout proliferating lipid-producing layers sebaceous glands (SGs) healthy acne-involved skin, we investigated effect SZ95 sebocytes how it alter changes induced palmitic acid (PA), major component with bioactive roles. We found that not only potent stimulator sebocyte proliferation, but also induces secretion interleukin (IL)6 down-regulates expression genes involved steroid retinoid metabolism. Importantly, when applied combination PA, PA-induced lipid accumulation was decreased secreted increased IL6 levels. Functional clustering differentially regulated treated EGF, PA or co-treated EGF+PA further confirmed be inducer hyperproliferative/inflammatory pathways (IL1 signaling), an being more pronounced presence PA. while group inflammatory up-regulated significantly treatment absence related homeostasis. Meta-analysis gene profiles whole acne tissue samples EGF- -treated showed co-activation implications disease. Altogether, our results reveal inflammation can modulated highlights need for system biological approaches better understand (immuno)biology.